Project Objectives
Pythium ultimum Whole Genome Sequencing Objectives
Our objectives are focused on generating sequence and annotation data for an economically important plant pathogen for which there is little genome sequence data available to date. Through this project, we will enable a range of scientists to pursue not only applied and basic research on Pythium, but also on other economically oomycete pathogens such as Phytophthora.
- Obj. 1: Sequence the genome of a significant plant pathogen, Pythium ultimum strain DAOM BR144 (=CBS805.95 and ATCC200006).
- Obj. 2: Annotate the genome for genes using not only an automated pipeline and targeted manual curation and by hosting experts in oomycete biology for an annotation workshop.
- Obj. 3: Generate skim sequence (2X coverage) second strain of P. ultimum to provide a resource for comparative genomics.
- Obj. 4: Train a graduate student in genomics and bioinformatics and engage scientists from multiple disciplines to annotate the Pythium genome.
Pythium ultimum EST Sequencing Objectives
- We will generate ESTs for Pythium ultimum to aid in generation of diagnostic markers and in annotation of the genome.
The Pythium ultimum EST Sequencing was supported by the USDA National Research Initiative Cooperative State Research Extension Education Service (grant 2006-55605-16645)
The Pythium ultimum Whole Genome Sequencing was supported by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service, grant number 2007-35600-17774 and 2007-35600-18886.
